Fig. 7
YBX1 is an important molecule of m5C regulating HGH1 expression in breast cancer. (A) YBX1 antibodies were used for RIP-qPCR to measure the level of YBX1 binding to HGH1 mRNA in the presence of NSUN2-WT. (B) Changes in the response to actinomycin D-mediated RNA synthesis inhibition and mRNA transcription interference after NSUN2 knockdown were detected. (C) RT-qPCR detection of the changes in the expression of the target gene HGH1 in the MCF7 and T47D cell lines after knockdown of siYBX1 and (D) overexpression of YBX1-WT and YBX1-Mut. (E) and (F) Western blot analysis of changes in protein levels. (G) and (H) RT-qPCR and Western blot experiments were performed to detect the synergistic effect of NSUN2 and YBX1 on the expression of the target gene HGH1. ns. no significant; Data are mean ± SD of three independent experiments. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 by t test