Fig. 5

HGH1 affects translation efficiency in BC cells. (A) STRING database analysis showing a protein interaction relationship between EEF2 and HGH1. (B) and (C) Volcano plot and histogram of LC-MS/TOF data showing the high expression of EEF2 and HGH1 in BC tissues. (D) and (E) MCF7 and T47D cells transfected with siEEF2 (D) or siHGH1 (E) were treated with 1 µM puromycin for 1 h, and whole-cell lysates were subjected to western blot analysis with an anti-puromycin antibody. (F) Proteins were used in large excess to capture EEF2 from MCF7 cells by the anti-HGH1-protein A/G magnetic beads complex. The eluted material was analyzed by western blot and immunoblotting with anti-EEF2, anti-HGH1, and anti-GAPDH antibodies. (G) The average translation efficiency (TE) of MCF7 cells was analyzed via Ribo-Seq. ns. no significant; Data are mean ± SD of three independent experiments for (C) and (G). **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 by t test