Fig. 4

Suppression of HGH1 delays the progression of breast cancer. (A) CCK-8 assays were used to detect BC cell proliferation rates after interference with siHGH1 or oeHGH1. (B) Colony formation experiments detected the colony formation ability of BC cells after interference with shHGH1 or oeHGH1. (C) Transwell assays were used to assess the migration and invasion ability of BC cells after HGH1 knockdown. (D) FITC and PE fluorescence dyes were used to detect changes in cell apoptosis by flow cytometry after HGH1 knockdown. (E) PI fluorescence dye was used to detect cell cycle alterations by flow cytometry upon HGH1 knockdown or HGH1 overexpression. (F) The mammary fat pads of BALB/c nude mice (n = 5 per group) were surgically injected into the mammary fat pads with MCF7 cells, the tumors were weighed, and the tumor volume was measured. (G) Representative images of HGH1 and Ki-67 expression in different groups of CDX tumors after IHC staining (all images 200×) ns. no significant; Data are mean ± SD of three independent experiments for (C-E). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 by t test