Fig. 2

NSUN2-mediated promotion of breast cancer progression relies on methyltransferase enzymatic activity. (A) and (B) Cellular proliferation rates of BC cells with NSUN2 knockdown, NSUN2-WT or NSUN2-DM determined by CCK-8 assays and (C) and (D) colony formation assays. (E) and (F) Transwell assays were used to investigate the cell migration and invasion ability of NSUN2-knockdown, NSUN2-WT or NSUN2-DM cells. (G) FITC and PE fluorescence dyes were used to detect changes in cell apoptosis after NSUN2 knockdown by flow cytometry. Data are mean ± SD of three independent experiments for (E-G). **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 by t test