Fig. 1

Higher NSUN2 expression in breast cancer samples. (A) NSUN2 expression in normal tissues and BC tissues from the TCGA dataset. (B) Plot of the overall survival probability of BC patients with high or low NSUN2 expression from the TCGA dataset. (C) NSUN2 mRNA expression in five clinical BC and adjacent tissue samples assessed by RNA-Seq (n = 5). (D) NSUN2 expression in five BC paraffin tissue samples. The samples were prepared and stained as described in the Materials and Methods (all images, 200×; n = 8). (E) LC-MS/TOF analysis of NSUN2 protein expression in five BC and adjacent tissue samples (n = 5). (F) Western blot analysis of NSUN2 expression in five breast cell lines. (G) The mammary fat pads of BALB/c nude mice (n = 5 per group) were surgically injected into the mammary fat pads with MCF7 cells, the tumors were weighed, and the tumor volume was measured. (H) Representative images of NSUN2 and Ki-67 expression in different groups of CDX tumors after IHC staining (all images, 200×). Data are mean ± SD (Standard deviation) of three independent experiments for (E) and (F). *p ≤ 0.05, **p ≤ 0.01, **p ≤ 0.001, ****p ≤ 0.0001 by t test of the indicated pairs or by log-rank (Mantel-Cox) test