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Fig. 6 | Breast Cancer Research

Fig. 6

From: Evaluating the immunologically “cold” tumor microenvironment after treatment with immune checkpoint inhibitors utilizing PET imaging of CD4 + and CD8 + T cells in breast cancer mouse models

Fig. 6

Autoradiography and immunofluorescence (IF) staining show that spatial heterogeneity and overall uptake are distinct measurements. A-B) Representative central slice of PET images showed high and low heterogeneity of [89Zr]Zr-DFO-CD4 and [89Zr]Zr-DFO-CD8 distribution, despite differences in total levels of uptake. C-D) Topographic map of [89Zr]Zr-DFO-CD4 and [89Zr]Zr-DFO-CD8 PET slices indicated regional hotspots. E-F) Corresponding autoradiography images immediately after PET imaging. G-H) Hematoxylin and eosin (H&E) staining of 4T1 tumor sections revealed that areas of high [89Zr]Zr-DFO-CD4 or [89Zr]Zr-DFO-CD8 uptake displayed a substantial concentration of densely packed tumor regions. I-J) IF staining for CD4 or CD8 in 4T1 tumor sections displayed a notable presence of CD4 + or CD8 + cells in proximity to high cellular tumor regions, a pattern consistent with the uptake pattern of the PET tracer

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Breast Cancer Research

ISSN: 1465-542X

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