Fig. 1
From: TRPS1 maintains luminal progenitors in the mammary gland by repressing SRF/MRTF activity
Ubiquitous TRPS1 depletion does not affect fitness a Overview of the genetic structure of the ubiquitous TRPS1 depletion model mouse line. The line harbors a CAG-rtTA3 transgene composed of CAG promoter followed the reverse tetracycline-controlled transactivator 3 (rtTA3) sequence. Sequences allowing the expression of a tGFP marker and Trps1 shRNA under the control of Tetracyclin Response Element (TRE) are inserted in the Col1a1 locus. Their expression is enabled by rtTA3 in the presence of doxycycline. b Immunoblot of mouse embryonic fibroblasts isolated from TRPS1-depletion lines showing the depletion of TRPS1 upon Dox-induced shRNA expression. Vinculin serves as a loading control. c Schematic of the phenotypic screening performed at the german mouse clinic. Animals used for the screening (left); males and females from shTrps1 #1 and #2 depletion lines and shRen #1 and #2 corresponding littermate controls. Right panel: short description of the screening pipeline. d X-rays pictures of the whole body, skull, arm, and leg from TRPS1 depletion mice and shRenilla littermate control mice. e Box plot of the bone marrow density of male and female animals of the indicated mouse lines. Females: shRen#1 n = 15, shTrps1#1 n = 14, shRen#2 n = 14, shTrps1#2 n = 9. Males: shRen#1 n = 15, shTrps1#1 n = 14, shRen#2 n = 15, shTrps1#2 n = 14. One-way ANOVA with Tukey HSD, post-hoc test. f Body weight of female animals from the indicated mouse lines during the 10 week-phenotypic screen. shRen#1 n = 15, shTrps1#1 n = 15, shRen#2 n = 15, shTrps1#2 n = 11. Two-way ANOVA, with Tukey HSD post-hoc test. g QS interval (ms) of male and female animals from each mouse line. Females: shRen#1 n = 15, shTrps1#1 n = 15, shRen#2 n = 15, shTrps1#2 n = 11. Males: shRen#1 n = 15, shTrps1#1 n = 14, shRen#2 n = 15, shTrps1#2 n = 15. Wilcoxon rank sum test. h Ejecton fraction (%) of male and female animals from each mouse line. Females: shRen#1 n = 15, shTrps1#1 n = 15, shRen#2 n = 15, shTrps1#2 n = 11. Males: shRen#1 n = 15, shTrps1#1 n = 14, shRen#2 n = 15, shTrps1#2 n = 15. Wilcoxon rank sum test Indicated p values are p.adj