Fig. 6

Combination therapy increases CD8 T cell cytotoxic activity and pro-inflammatory cytokine production. Spleens from untreated tumor-bearing and treated mice were isolated seven days after treatment as outlined in Fig. 5A and dispersed into single cell suspensions. (A) CD8⁺ T cells were isolated by magnetic bead purification and cocultured 1:1 with Oregon green-labelled 4T1 cells. Cells were stained with Annexin V and 7AAD after 18-hour to determine cytotoxic activity against 4T1 cells. (B, C) Supernatants were collected from the 18-hour cocultures to measure cytokine production. Concentrations of (B) IFNγ and (C) TNF were determined by ELISA (n = 3 per group). ^*p < 0.05 compared to untreated, ^†p < 0.05 compared to VSV-GFP, ^‡p < 0.05 compared to VSV-p14, ^§p < 0.05 compared to VSV-p15, ^¶p < 0.05 compared to VSV-GFP + glycolipid-loaded DCs