Fig. 4

VSV-FAST constructs increase immune infiltration and activation in 4T1 tumors. Mice were treated as described in Fig. 2A. Twenty-four hours after the final virus treatment, tumors were isolated and dispersed into single cell suspensions. (A-B) Flow cytometry was used to assess immune cell infiltration (n = 8–9 per group). The number of (A) NKT cells (CD1d tetramer⁺ TCRβ⁺), (B) NK cells (NK1.1⁺ TCRβ⁻) (C) CD8⁺ T cells (TCRβ⁺ CD8α⁺), (D) CD4⁺ T cells (TCRβ⁺ CD4⁺) and the expression of CD69, PD-1, and intracellular IFNγ by these subsets was assessed. (E) The number of dendritic cells (MHC II⁺ CD11c⁺) and CD80 expression was also examined.^*p < 0.05 compared to untreated, ^†p < 0.05 compared to VSV-GFP