Fig. 5
From: PTHrP intracrine actions divergently influence breast cancer growth through p27 and LIFR
Truncated. PTHrP induces CDKN1B in the bone metastatic site, but enhances osteolysis and tumor burden. (A) qPCR analysis for CDKN1B (p27) normalized to ACTB as a marker of total tumor burden in the bone marrow of mice inoculated with MSCV, FLSEC, DNLS, or DNLS + CTERM cells via intracardiac injection. n = 8–10 mice/group. (B-D) Total osteolytic lesion area and lesion number (per mouse) based on radiographic analyses for mice described in A. White arrows indicate osteolytic lesions. (E) Flow cytometric quantitation of percent CD298 + tumor cells in the bone marrow of mice described in A. (F) qPCR analysis for RANKL/OPG (Tnfsf11 / Tnfrsf11b) in whole homogenized femurs from mice described in (A). n = 8–10 mice/group. *p < 0.05, **p < 0.01, or ****p < 0.0001 vs. MSCV by one-way with multiple comparisons. Graphs represent mean ± SEM